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College of Medicine

About

Ph.D. (1990) University of Waterloo

B.Sc., (Honours) (1986) University of Waterloo

Our laboratory is interested in the expression and function of heat shock genes during embryonic development of the zebrafish, a model system which is used extensively to examine the molecular mechanisms that regulate embryonic development.

Specific projects are aimed at elucidating the roles that specific heat shock proteins play both in normal embryonic development, and in the protection of rapidly developing embryonic cells from different forms of environmental stress.

Other projects are developing a transgenic model whereby heat shock gene expression can be used as a toxicological biomarker in live fish embryos, and using synchrotron technologies to examine tissue-specific localization of metals in embryonic zebrafish.

Selected Publications

  • Korbas M, Lai B, Vogt S, Gleber SC, Karunakaran C, Pickering IJ, Krone PH, George GN.  Methylmercury targets photoreceptor outer segments.  ACS Chem Biol. 2013 Oct 18;8(10):2256-63. doi: 10.1021/cb4004805. Epub 2013 Aug 19.
  • Swan CL, Evans TG, Sylvain N, Krone PH.  Zebrafish HSF4: a novel protein that shares features of both HSF1 and HSF4 of mammals.  Cell Stress Chaperones. 2012 Sep;17(5):623-37. doi: 10.1007/s12192-012-0337-3. Epub 2012 Apr 17.
  • Korbas M, Macdonald TC, Pickering IJ, George GN, Krone PH.Chemical Form Matters: Differential Accumulation of Mercury Following Inorganic and Organic Mercury Exposures in Zebrafish Larvae.  ACS Chem Biol. 2011 Nov 7. [Epub ahead of print]
  • Taherian A, Ovsenek N, Krone PH.  Expression of hsp90 alpha and hsp90 beta during Xenopus laevis embryonic development.  Iran Biomed J. 2010 Oct;14(4):127-35.
  • Korbas M, Krone PH, Pickering IJ, George GN. Dynamic accumulation and redistribution of methylmercury in the lens of developing zebrafish embryos and larvae. J Biol Inorg Chem. 2010 Sep;15(7):1137-45. Epub 2010 May 30.
  • Korbas M, Blechinger SR, Krone PH, Pickering IJ, George GN. Localizing organomercury uptake and accumulation in zebrafish larvae at the tissue and cellular level. Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12108-12. Epub 2008 Aug 21.
  • Taherian A, Krone PH, Ovsenek N.  A comparison of Hsp90alpha and Hsp90beta interactions with cochaperones and substrates.  Biochem Cell Biol. 2008 Feb;86(1):37-45.
  • Kusch R, Krone P, Chivers D. Chronic exposure to low concentrations of water-borne cadmium during embryonic and larval development results in the long-term hindrance of anti-predator behaviour in zebrafish. Environ Toxicol Chem. 2008 Mar;27(3):705-10.
  • MacKay AB, Mhanni AA, McGowan RA, Krone PH. Immunological detection of changes in genomic DNA methylation during early zebrafish development. Genome. 2007 Aug;50(8):778-85.
  • Matz CJ, Krone PH. Cell death, stress-responsive transgene activation, and deficits in the olfactory system of larval zebrafish following cadmium exposure.Environ Sci Technol. 2007 Jul 15;41(14):5143-8
  • Blechinger SR, Kusch RC, Haugo K, Matz C, Chivers DP, Krone PH. Brief embryonic cadmium exposure induces a stress response and cell death in the developing olfactory system followed by long-term olfactory deficits in juvenile zebrafish. Toxicol Appl Pharmacol. 2007 Oct 1;224(1):72-80. Epub 2007 Jul 17.
  • Chivers DP, Wisenden BD, Hindman CJ, Michalak TA, Kusch RC, Kaminskyj SG, Jack KL, Ferrari MC, Pollock RJ, Halbgewachs CF, Pollock MS, Alemadi S, James CT, Savaloja RK, Goater CP, Corwin A, Mirza RS, Kiesecker JM, Brown GE, Adrian JC Jr, Krone PH, Blaustein AR, Mathis A. Epidermal 'alarm substance' cells of fishes maintained by non-alarm functions: possible defence against pathogens, parasites and UVB radiation.Proc Biol Sci. 2007 Oct 22;274(1625):2611-9.
  • Evans TG, Belak Z, Ovsenek N, Krone PH. Heat shock factor 1 is required for constitutive Hsp70 expression and normal lens development in embryonic zebrafish. Comp Biochem Physiol A Mol Integr Physiol. 2007 Jan;146(1):131-40. Epub 2006 Oct 6.
  • Matz CJ, Treble RG, Krone PH. Accumulation and elimination of cadmium in larval stage zebrafish following acute exposure. Ecotoxicol Environ Saf. 2007 Jan;66(1):44-8. Epub 2006 Jan 10.
  • Evans, T.G., Y. Yamamoto, W.R. Jeffery, and P.H. Krone. 2005. Zebrafish Hsp70 is required for embryonic lens formation. Cell Stress and Chaperones 10, 66-78.
  • Krone, P.H., S.R. Blechinger, T.G. Evans, J.A. Ryan, E.J. Noonan, and L.E. Hightower. 2005. Use of fish liver PLHC-1 cells and zebrafish embryos in cytotoxicity assays. Methods 35, 176-187.
  • Blechinger, S.R., J.T. Warren, J.Y. Kuwada, and P.H. Krone. 2002. Developmental toxicology of cadmium in living embryos of a stable transgenic zebrafish line. Environmental Health Perspectives 110, 1041-1046.
  • Martin, C.C., C.H. Tsang, R.G. Beiko, and P.H. Krone. 2002. Expression and genomic organization of the zebrafish chaperonin gene complex. Genome 45, 804-811.
  • Blechinger, S.R., T.G. Evans, P.T. Tang, J.Y. Kuwada, J.T. Warren Jr., and P.H. Krone. 2002. The heat-inducible zebrafish hsp70 gene is expressed during normal lens development under non-stress conditions. Mechanisms of Development 112, 213-215.
  • Willey, J.B. and P.H. Krone. 2001. Effects of endosulfan and nonylphenol on the primordial germ cell population in pre-larval zebrafish embryos. Aquatic Toxicology 54, 113-123.
  • Martin, C.C., P.-T. Tang, G. Barnardo, and P. H. Krone. 2001. Expression of the chaperonin 10 gene during zebrafish development. Cell Stress and Chaperones 6, 38-43.
  • A. Adam, R. Bartfai, Z. Lele, P.H. Krone, and L. Orban. 2000. Heat-inducible expression of a reporter gene detected by transient assay in zebrafish. Experimental Cell Research, 256, 282-290.
  • M.C. Halloran, M. Sato-Maeda, J.T. Warren, F. Su, Z. Lele, P.H. Krone, J. Kuwada, and W. Shoji. 2000. Laser-induced gene expression in specific cells of transgenic zebrafish. Development, 127, 1953-1960.
  • J.B. Sass, C. C. Martin, and P.H. Krone. 1999. Restricted expression of the zebrafish hsp90a gene in slow and fast muscle fibre lineages. International Journal of Developmental Biology, 43, 835-838. (Figure from publication chosen as cover photo for this issue of journal).
  • Z. Lele, S.D. Hartson, C.C. Martin, L. Whitesell, R.L. Matts and P.H. Krone, 1999. Disruption of zebrafish somite development by pharmacologic inhibition of Hsp90. Developmental Biology, 210: 56-70.
  • Z. Lele, S. Engel and P. H. Krone, 1997. Hsp47 and hsp70 gene expression is differentially regulated in a stress and tissue specific manner in zebrafish embryos. Developmental Genetics, 21: 123-133.
  • Z. Lele and P. H. Krone, 1997. Expression of genes encoding the collagen-binding heat shock protein (Hsp47) and type II collagen in developing zebrafish embryos. Mechanisms of Development, 61, 89-98.
  • J.B. Sass and P. H. Krone, 1997. Hsp90a gene expression may be a conserved feature of vertebrate somitogenesis. Experimental Cell Research, 233, 391-394.