- Evolutionary developmental biology
- Physical anthropology
- Craniofacial/dental morphology and evolution
Professor, Anatomy, Physiology & Pharmacology, University of Saskatchewan.
Ph.D. Anatomy, Department of Anatomy and Developmental Biology, University College London.
B.Sc. Biological Anthropology, Department of Anthropology, University of Toronto.
My research background is in physical anthropology, broadly, the study of primate biology and evolution. Over time I’ve morphed into an evolutionary developmental biologist and have worked with various model organisms, including mouse, chick and snake.
Perhaps because I am one, primates remain close to my scientific heart. While I use mouse models in much of my work, I typically ask scientific questions that I hope will inform some aspect of human evolution. In particular, I work to better understand primate craniofacial evolution and development.
Recently, I was the first recipient of the Science Communication & Public Engagement Award (2023) from the American Association for Anatomy. I organized Saskatoon's 2017 and 2018 March for Science YXE Events. Currently, I organize Café Scientifique-Saskatoon. Previously, I co-founded and co-organized one of the first Canadian Café Scientifiques in Vancouver, BC (est. 2004) with then fellow postdoc, Anne Mullin. In 2005, I created a home-grown science and technology radio show called “My Science Project” that aired on CiTR 101.9 FM UBC campus radio until 2008. Anne and I shared broadcasting duties as producers and hosts. In August 2009 I was fortunate enough to participate in the 2-week Science Communications Program at The Banff Centre. For me science outreach is both fun and crucial: I continue to be on the look-out for ways to fill the world with more science.
In service to my research community, I serve on the Steering Committee of the Biological Anthropology Women's Mentoring Network. I also serve on the Editorial Boards of Developmental Dynamics and JEZ-B. Recently, I have also served on the Board of Directors of the American Association for Anatomy (2019-2022) and on the Executive of the Pan-American Society for Evolutionary Developmental Biology (2018-2019). From 2016-2019, I served as an Evaluation Group member for NSERC's Discovery Grant program. I continue to do ad hoc grant reviews for NSERC and for international science funding agencies (e.g., NSF). I was honoured to be nominated as a 2022 Distinguished Graduate Mentor by my trainees and to then be recognized with this award by the University of Saskatchewan.
My laboratory’s principal research question is, “What underlies the coordinated development and evolution of the jaws and teeth?” We combine genetic, morphological, statistical shape analyses, and high-resolution image data to get at the mechanisms that coordinate pre- and postnatal developmental changes among faces, jaws and teeth. My research and expertise have been featured locally, nationally and internationally on the The New Human episode of CBC's The Nature of Things with David Suzuki (teaser clip), CBC News, CBC Radio 2 Drive, The Star Phoenix, National Geographic, Gizmodo.com, Fusion.net, The Conversation Canada, and HealthyDebate.ca, among other media outlets.
Evolutionary Developmental Biology of the Teeth and Jaws
As teeth and jaws grow they must fit together to work properly and allow an animal to eat. Because teeth sit within the jawbone, any developmental or evolutionary changes in jaw shape or size will invariably affect the teeth and vice versa. To understand how a body part develops is to understand how it may have evolved. It is not known how the timing of tooth and jaw development is coordinated such that these parts “fit” together properly during pre- and postnatal growth. With support from NSERC Canada, our current focus is to explain how the pace of tooth and jaw development has evolved to be (relatively) synchronous.
One option is that the timing of tooth development is controlled by signals from the jaw tissues, and vice versa. However, good evidence suggests that the opposite is true: the pace of tooth and jaw development has been naturally selected to run in parallel with no cross-talk between tooth and jaw tissues. We aim to tackle this research question using complimentary genetic, statistical and advanced 3D imaging data alongside mammalian models at various stages of pre- and postnatal development.
Being virtually next door to the Canadian Light Source, home to Canada’s only Synchrotron, my lab has access to amazingly powerful high-resolution imaging technologies with which to study morphology. Supported by the Canadian Foundation for Innovation, we have an Optical Projection Tomography scanning system, enabling us to image and measure embryonic morphology alongside the genetics driving this development. We are also fortunate to have the Computed-Tomography Facility at our department in Dr. Cooper's Lab.
The Master Plan
Our aim is to start to fill a fundamental gap in scientific knowledge by helping to explain how jaw and tooth development is coordinated in time. This insight should further clarify how teeth and jaws have developed and evolved such an amazing variety of different yet functional forms across living and extinct mammals. More broadly, this insight also speaks to how functionally-linked body parts develop and evolve in tandem.
My research is supported by the Natural Sciences and Engineering Research Council of Canada (NSERC), the Canadian Foundation for Innovation (CFI),
Peer-reviewed Research Articles Underlined = my trainee
- Green RM, Lo Vercio L, Dauter A, Barretto EC, Devine J, Garcia MV, Marchini M, Robertson S, Zhao X, Mahika A, Shakir MB, Guo S, Boughner JC, Dean W, Lander AD, Marcucio RS, Forkert ND Hallgrimsson B (bioRxiv, 2023.05. 12.540515) Quantifying the relationship between cell proliferation and morphology during development of the face.
- Van Ankum EM, Majcher K, Dolovich A, Flegel KP, Johnston JD, Boughner JC (In press) Food texture and vitamin D influence mouse mandible form and molar roots. The Anatomical Record
- Bolter D, Cameron N, Churchill S, Hawks J, Berger L, Bernstein R, Boughner JC, Elton S, Leece AB, Mahoney P, Molopyane K, Monson T, Pruetz J, Schell L, Stull K, Wolfe C (2023) Addressing the growing fossil record of sub-adult hominins by reaching across disciplines. Evolutionary Anthropology 32(4):180-184.
- Boughner JC*, Marchiori DF*, Packota GV. 2021. *Co-corresponding authors. Unexpected variation in human molar size patterns. Journal of Human Evolution. 161C:103072. PubMed
- Ko D, Uppal J, Kelly T, Thompson L, Rostampour N, Webb MA, Xiu N, Belev G, Mondal P, Cooper DML, Boughner JC. 2021. Timing of mouse molar formation is independent of jaw length including retromolar space. Journal of Developmental Biology Special Issue Craniofacial Genetics & Developmental Biology. 9(1):8. DOI.org/10.3390/jdb9010008 FullArticle
- Ziermann J, Estevez-Altava B, Diogo R, Boughner JC. 2021. Anatomical comparison across heads, fore- and hindlimbs in mammals using network models. Journal of Anatomy. 239(1):12-31. PubMed
- Papakyrikos AM, Arora M, Austin C, Boughner JC, Capellini T, Dingwall H, Greba Q, Howland JG, Kato A, Wang X-P, Smith TM. 2020. Biological clocks and incremental growth line formation in dentine. Journal of Anatomy. 237(2):367-378 doi:10.1111/joa.13198. PubMed
- Marchiori DF, GV Packota, JC Boughner. 2019. Initial third molar development is delayed in jaws with short distal space: An early impaction sign? Archives of Oral Biology 106;104475. PubMed
- Rostampour N, Appelt CM, Abid A, Boughner JC. 2019. Expression of new genes in vertebrate tooth development and p63 signaling. Developmental Dynamics 248(8):744-755. PubMed
- Boughner JC. 2018. Bad molars? The origins of wisdom teeth. The Conversation Canada, November 8th. FullArticle
- Phen A, J Greer, J Uppal, J Der, JC Boughner. 2018. Upper jaw development in the absence of teeth: new insights for craniodental evo-devo integration. Evolution & Development 20(5):146-159. PubMed
- Boughner JC, MC van Eede, S Spring, LX Yu, N Rostampour, RM Henkelman. 2018. P63 expression plays a role in developmental rate, embryo size, and local morphogenesis. Dev Dyn. 247(5):779-787. PubMed
- Boughner JC. 2017. Implications of vertebrate craniodental Evo-Devo for human oral health. J. Experimental Zoology: Part B Molecular & Developmental Evolution. doi: 10.1002/jez.b.22734. 328(4):321–33 html
- Raj MT, JC Boughner. 2016. Detangling the evolutionary developmental integration of dentitions and jaws: a p63-regulated gene network for odontogenesis exclusive of mandible morphogenesis. Evolution & Development 18(5-6): 317-323. html
- Marchiori DF, GV Packota, JC Boughner. 2016. Third molar mineralization as a function of available space. Acta Odontologica Scandanivica. 74(7):509-517. html
- M Linde-Medina, JC Boughner, S Santana, R Diogo. 2016. Are more diverse parts of the mammalian skull more labile? Ecology & Evolution 6(8): 2318-24.
- Wong MD, CE Matthijs, S Spring, S Jevtic, JC Boughner, JP Lerch, and RM Henkelman. 2015. 4D atlas of the mouse embryo for precise morphological staging. Development 142:3583-91.
- Diogo R, B Esteve-Altava; C Smith; JC Boughner; D Rasskin-Gutman. 2015. Anatomical Network comparison of human upper and lower, newborn and adult, and normal and abnormal limbs, with notes on development, pathology and limb serial homology vs. homoplasy. PLOS ONE 10(10):e0140030.
- Boughner JC, J Der, KL Kuykendall. 2015. A multivariate approach to dentally age free-lived and captive-raised chimpanzees (P. troglodytes). Am J Phys Anthropol. 158(3):452-462.
- Esteve-Altava B, Boughner JC, Diogo R, Villmoare BA, Rasskin-Gutman D. 2015. Anatomical Network Analysis shows decoupling of modular lability and complexity in the evolution of the Primate skull. PLOS ONE 10(5): e0127653.
- Esteve-Altava B, R Diogo, C Smith, JC Boughner, D Rasskin-Gutman. 2015. Anatomical networks, musculoskeletal evolution and human anatomy and modularity. Nature Scientific Reports 5, 8298; DOI:10.1038/srep08298
- Raj MT, M Prusinkiewicz, DML Cooper, G Belev, MA Webb, JC Boughner. 2014. Imaging earliest tooth development in 3D using a new silver-based tissue contrast agent. The Anat. Rec. 297(2):222-33. doi: 10.1002/ar.22845.
- Boughner JC. 2013. Maintaining Perspective on Third Molar Extraction. J Cdn Dental Assoc. 9(6):347-49 (79:d106. http://www.jcda.ca/article/d106).
- Paradis MR, MT Raj, JC Boughner. 2013. Jaw growth in the absence of teeth: the developmental morphology of edentulous mandibles using the p63 mouse mutant. Evol & Dev.15(4):268-79 (DOI: 10.1111/ede.12026). html
- Boughner JC, MC Dean, C Wilgenbusch. 2012. Permanent tooth mineralization in bonobos (Pan paniscus) and chimpanzees (P. troglodytes). Am J Phys Anthropol. 149(4): 560-71. html
- Parsons TE, EJ Schmidt, JC Boughner, HA Jamniczky, RS Marcucio, B Hallgrímsson. 2011. Epigenetic integration of the developing brain and face. Dev Dyn. 240: 2233–44.pdf
- Boughner JC. 2011. Making space for permanent molars in growing baboon (Papio anubis) and great ape (Pan paniscus and P. troglodytes) mandibles: Possible ontogenetic strategies and solutions. Anat Res Int 2011:16pp. Article ID 484607. doi:10.1155/2011/484607pdf
- Meruvia-Pastor O, J Soh, EJ Schmidt, JC Boughner, M Xiao, HA Jamniczky, B Hallgrimsson, C Sensen. 2011. Estimating Cell Count and Distribution in Labeled Histological Samples Using Incremental Cell Search. Int J of Biomed Imaging vol. 2011: 16pp. Article ID 874702. doi:10.1155/2011/874702 html
- Schmidt EJ, TE Parsons, HA Jamniczky, J Gitelman, C Trpkov, JC Boughner, C Logan, CW Sensen, B Hallgrímsson. 2010. Micro-computed tomography-based phenotypic approaches in embryology: Sources and effects of procedural artifacts on assessments of embryonic craniofacial growth and development. BMC Dev Biol10:18-32. html
- Jamniczky HA, JC Boughner, PN Gonzalez, CD Powell, C Rolian, EJ Schmidt, TE Parsons, FL Bookstein, B Hallgrímsson. 2010. Mapping the Epigenetic Landscape: Rediscovering Waddington in the Post-Genomic Age.BioEssays 32:1-6. pdf
- Hallgrímsson B, HA Jamniczky, NM Young, C Rolian, TE Parsons, JC Boughner, R Marcucio. 2009. Deciphering the Palimpsest: Studying the relationship between morphological integration and phenotypic covariation. Evol Biol36: 355-76. pdf
- Boughner JC, B Hallgrímsson. 2008 Biological spacetime and the temporal integration of functional modules: a case study of dento-gnathic developmental timing. Dev Dyn 237: 1-17. Featured in “Highlights in DD”, June 2008. pdf
- Boughner JC, MC Dean. 2008. Mandibular shape, ontogeny and dental development in bonobos (Pan paniscus) and chimpanzees (Pan troglodytes). Evol Biol. 35: 296-308. pdf
- Boughner JC*, S Wat*, VM Diewert, NM Young, LW Browder, B Hallgrímsson. 2008. Short faced mice and developmental interactions between the brain and the face. J Anat 213: 646-62. pdf
- Boughner JC*, M Buchtová*, K Fu, VM Diewert, B Hallgrímsson, JM Richman. 2007. Embryonic development ofPython sebae I. Staging criteria & macroscopic skeletal morphogenesis of the head & limbs. Zoology 110: 212-30. pdf
- Buchtová M, Boughner JC, K Fu, VM Diewert, JM Richman. 2007. Embryonic development of Python sebae II. Craniofacial microscopic anatomy, cell proliferation & apoptosis. Zoology 110: 231-51. html
- Richman JM, M Buchtová, JC Boughner. 2006. Comparative ontogeny & phylogeny of the upper jaw skeleton in amniotes. Dev Dyn 235: 1230-43. pdf
- Boughner JC, MC Dean. 2004. Does space in the jaw influence timing of molar crown initiation? A model using baboons (Papio anubis) and great apes (Pan troglodytes, Pan paniscus). J Hum Evol 46: 253-75. html
- Understanding Human Anatomy and Pathology: an Evolutionary Developmental Guide for Medical Students. 2016. R Diogo, D Noden, C Smith, J Molnar, JC Boughner, C Barrocas, J Bruno. Taylor & Francis Group: Boca Raton.
- Developmental Approaches to Human Evolution. 2016. JC Boughner & CP Rolian (Eds). This book (invited by Wiley) showcases leading-edge research in Evolutionary Developmental Anthropology. Wiley: Hoboken.
- Appelt CM, Van Ankum EM, Marchiori DF, Boughner JC. 2021. Cell processes in the evolution of jaws and teeth in primates. Invited book chapter. In J. Richtsmeier & K. Pitirri (Eds.), Volume 1. Evolutionary Cell Processes, Primate Skin, Energetics and Feeding. Chapter 3. (S. Moody, B.K. Hall (Eds.), Evolutionary Cell Processes series). New York: CRC Press.
- Marchiori DF, GV Packota, Boughner JC. 2019. Three dimensional assessment of crown size and eruption space for developing third molars: Research techniques based on cone-beam computed tomography (CBCT). Invited book chapter. In P. Papagerakis (Ed.), Methods in Molecular Biology: Odontogenesis. Springer. (pp. 341-356) Clifton, NJ: Springer.
- Boughner JC, DML Cooper. 2019. Silver-albumin tissue staining protocol to visualize odontogenesis in whole embryos. Invited book chapter. In P. Papagerakis (Ed.), Methods in Molecular Biology: Odontogenesis. Springer. (pp. 197-210) Clifton, NJ: Springer.
- Boughner JC. 2016. The tooth of the matter: the Evo-Devo of phenotypic change. Developmental Approaches to Human Evolution. JC Boughner, CP Rolian (Eds). pp.35-60. Wiley: Hoboken.
- CP Rolian, Boughner JC. 2016. Introduction to Evo-Devo-Anthro. Developmental Approaches to Human Evolution.Boughner JC, CP Rolian (Eds). pp.1-15. Wiley: Hoboken.
- Boughner JC. 2010. The evolution of the vertebrate jaw: How would you eat without one? Biology on the Cutting Edge.SL Gillies, S Hewitt (Eds). Supplement to Biology. 10th Edition. Campbell NA, Reece JB. Pearson Canada: Toronto. pp. 85-90.
- Hallgrímsson B, Boughner JC, AL Turinsky, TE Parsons, C Logan, CW Sensen. 2009. Geometric morphometrics and the study of development. Advanced Imaging in Biology & Medicine: Technology, Software Environments & Applications. CW Sensen & B Hallgrímsson (Eds). Springer Verlag: Berlin. pp. 319-38.
- Meruvia-Pastor O, J Soh, M Xiao, E Schmidt, C Logan, Boughner JC, N Jones, D Osborn, J Santiago, J Gittleman, B Hallgrímsson, CW Sensen. 2009 Fast Interactive Integration of Cross-Sectional Image Datasets and Surface Data for Morphometric Analysis. Medicine Meets Virtual Reality 17, NextMed: Design for the Well Being. JD Westwood, S Westwood, RS Haluck, H Hoffman, GT Mogel, R Phillips, RA Robb, KG Vosburgh (Eds). IOS Press: Amsterdam. pp. 183-86.
- Boughner JC, MC Dean. 2005. Mind the gap: Creating space for the permanent molars in the developing primate mandible. Current Trends in Dental Morphology Research. E Żądzińska (Ed). University of Lodz: Lodz, Poland. pp. 211-22.