Picture of Sidney Hayes

Sidney Hayes Professor Biochemistry, Microbiology & Immunology

Address
1B35 Health Sciences

Research Area(s)

  • Small RNA Silencing of Replication and Gene Expression; Phage Display Development

About

Areas of Expertise

Molecular Genetics, Microbial Physiology

Research Interests

A)  Small RNA Silencing of Replication and Gene Expression: bacteriophage lambda (λ) replication initiation silencing, influence of λ replication associated proteins on λ and coli metabolism, e.g., influence of λ gpP on DnaB helicase sequestration and cell mutation; OOP RNA silencing of cII.

B)  Phage Display Development: Platform genetics system for vaccine construction using phage lambda as a display particle for foreign antigens; to appraise molecular interactions & obstacles to generating high display density, high yield bacteriophage lambda display particles (LDP) for use in phage display vaccines and for phage therapy alternatives.

Recent Publications

Hayes S. Bacterial Virus Lambda Gpd-Fusions to Cathelicidins, α- and β-Defensins, and Disease-Specific Epitopes Evaluated for Antimicrobial Toxicity and Ability to Support Phage DisplayViruses. 2019;11(9):869. Published 2019 Sep 17. doi:10.3390/v11090869 Supplement File

Hayes S, Rajamanickam K, Hayes C. Complementation Studies of Bacteriophage λ O Amber Mutants by Allelic Forms of O Expressed from Plasmid, and O-P Interaction PhenotypesAntibiotics (Basel). 2018;7(2):31. Published 2018 Apr 5. doi:10.3390/antibiotics7020031

Rajamanickam K, Hayes S. The Bacteriophage Lambda CII Phenotypes for Complementation, Cellular Toxicity and Replication Inhibition Are Suppressed in cII-oop Constructs Expressing the Small RNA OOP. Viruses. 2018;10(3):115. Published 2018 Mar 7. doi:10.3390/v10030115

Cano, P.G., Gamage, L.N.A., Marciniuk, K., Hayes, C., Napper, S., Hayes, S., and Griebel, P.J. (2017) Lambda display phage    as a mucosal vaccine delivery vehicle for peptide antigens.  Vaccine 35, 7256-7263

Hayes, S. (2014) Two patents, issued in USA:(no. 8,663,913 B2) and Canada (no. 2,761,105) on platform genetic system for phage display vaccine production

Hayes S, Wang W, Rajamanickam K, Chu A, Banerjee A, and Hayes C.  Lambda gpP-DnaB Helicase Sequestration and gpP-RpoB Associated Effects: On Screens for Auxotrophs, Selection for RifR, Toxicity, Mutagenicity, Plasmid Curing.  Viruses. 2016, 8(6),172;doi:10.3390/v8060172

Hayes S, Erker C, Horbay MA, Marciniuk K, Wang W, Hayes C.  Phage Lambda P protein: trans-activation, inhibition phenotypes and their suppression.  Viruses. 2013 Feb 6;5(2):619-53

Hayes S, Horbay MA, Hayes C (2012) A CI-Independent Form of Replicative Inhibition: Turn Off of Early Replication of Bacteriophage Lambda. PLoS ONE 7(5): e36498. doi:10.1371/journal.pone.0036498

Hayes S, Gamage LN, Hayes C. Dual expression system for assembling phage lambda display particle (LDP) vaccine to porcine Circovirus 2 (PCV2). Vaccine. 2010 Sep 24;28(41):6789-99. Epub 2010 Aug 1.

Gamage LN, Ellis J, Hayes S. Immunogenicity of bacteriophage lambda particles displaying porcine Circovirus 2 (PCV2) capsid protein epitopes. Vaccine. 2009 Nov 5;27(47):6595-604. Epub 2009 Aug 25.

Hayes S, Asai K, Chu AM, Hayes C. NinR- and red-mediated phage-prophage marker rescue recombination in Escherichia coli: recovery of a nonhomologous immlambda DNA segment by infecting lambdaimm434 phages.Genetics. 2005 Aug;170(4):1485-99. Epub 2005 Jun 14.

Hayes S, Slavcev RA. Polarity within pM and pE promoted phage lambda cI-rexA-rexB transcription and its suppression. Can J Microbiol. 2005 Jan;51(1):37-49.

Slavcev RA, Hayes SOver-expression of rexA nullifies T4rII exclusion in Escherichia coli K(lambda) lysogens. Can J Microbiol. 2004 Feb;50(2):133-6.

Slavcev RA, Hayes SBlocking the T4 lysis inhibition phenotype. Gene. 2003 Dec 4;321:163-71.

Slavcev RA, Bull HJ, Hayes SBacteriophage lambda repressor allelic modulation of the Rex exclusion phenotype.Can J Microbiol. 2003 Mar;49(3):225-9.

Slavcev RA, Hayes SStationary phase-like properties of the bacteriophage lambda Rex exclusion phenotype. Mol Genet Genomics. 2003 Apr;269(1):40-8. Epub 2003 Mar 5.

Dimmock JR, Zello GA, Vashishtha SC, Hayes SJ, Mutalik S, Kumar S, Venkatesh M, Udupa N. A toxicological evaluation of 2-dimethylaminomethyl-1-phenyl-2-propen-1-one hydrochloride. Pharmazie. 2003 Feb;58(2):136-9.

Slavcev RA, Hayes SRex-centric mutualism. J Bacteriol. 2002 Feb;184(3):857-8.